Contribución INIDEP 2450
Isla Naveira, Rocío; Hollmann, Gabriela; Monserrat, José María; de Souza Votto, Ana Paula; da Silveira, Julie Medeiros; Matsumoto, Andressa M.; Zortéa, Lais; Taipe Huisa, Andy Joel; Massa, Agueda E.; Churio, María Sandra. (2025).

Exploring natural sunscreens: UVB protection and antioxidant properties in gadusol-rich fish roes extracts. Photochemistry and Photobiology
https://doi.org/10.1111/php.70042

Abstract
The search for natural alternatives to synthetic sunscreens has driven interest in marine compounds with antioxidant and UV-protective properties. The present study expands our understanding of the potential of gadusol by evaluating the photochemoprotective and antioxidant effects of extracts from an underexploited marine by-product: the roes of yellowtail amberjack fish (Seriola lalandi). UVB-mediated responses were studied in vitro and in vivo using HaCaT keratinocytes and Caenorhabditis elegans worms, respectively. Additionally, several antioxidant assays were conducted to evaluate the antioxidant capacity and thermal stability over time. We also tested the docking binding of gadusol to the Nrf2-binding domain of Keap1 to better understand its potential chemoprotective role. Overall, the gadusol-containing extracts exhibited remarkable stability over time, offering effective protection against UVB radiation in both in vitro and in vivo models. This information contributes to a better characterization of the functional role of gadusol in crude extracts and its relevance for the design of innovative applications in various pharmaceutical and cosmetic industries.

Contribución INIDEP 2454
Dejesús Prieto Garcete; María Rebeca; Alcolea Ersinger, Victoria F.; Vittone, Marina; Dell'Erba, Ignacio M.; Cativa, Nancy M.; Lamas, Daniela. (2025).

Effect on protein content and digestibility of traditional bread by incorporating Arthrospira platensis. Journal of Applied Phycology 37(5):3609-3620
https://doi.org/10.1007/s10811-025-03597-8

Abstract
Marine Environmental Research212Since the world population is expected to reach 9.8 billion by 2050, a global demand for alternative proteins to traditional ones is expected. Alternative plant-based proteins are experiencing significant global demand as they offer similar characteristics, at a relatively more affordable cost than animal proteins. In recent years, the industrial production of microalgae biomass has received attention attributable to its rich content of quality proteins. On the other hand, due to their low carbon footprint, wastewater treatment benefits and carbon credit from industrial CO2 conversion, microalgae-based proteins have the potential to provide ecologic benefits. In this sense, the study of new species of protein-rich microalgae is an area of ongoing research. Thus, the main objective of the present study was to culture and characterize microalgal isolates of Arthrospira platensis from the Yaragui Lagoon of the Paraguayan Chaco. Nitrogen protein was studied by Kjeldahl method and the same technique was applied to obtain the non-protein nitrogen, but after treatment of the sample with trichloroacetic acid to remove proteins. The hydrosoluble protein content was measured by the Lowry method after freezing and thawing followed by sonication, and was compared with the Kjeldahl method. The nitrogen-to-protein conversion factor for biomass experimentally obtained was 6.2508 for nitrogen measured by Kjeldahl. Protein total content was around 67 percent and the hydrosoluble fraction corresponds to 66 percent of it. The proximate composition, the protein profile, the molecular structure and functional groups were also studied. Finally, the addition of Arthrospira to bread showed that nutritional quality was improved and the level of protein digestibility achieved was satisfactory. Thus, the results suggest that using A. platensis from the Yaragui Lagoon of the Paraguayan Chaco to make rich protein products provides a viable alternative for human consumption.

Contribución INIDEP 2455
Alcolea Ersinger, Victoria F.; Lamas, Daniela; Massa, Agueda E. (2025).

A review of marine collagens: approaches on extractions, applications, market, and future trends. Environmental Science and Pollution Research 32:16077-16097
https://doi.org/10.1007/s11356-025-36684-x

Abstract
Collagen obtained from the fishing industry is a promising alternative source that utilizes by-products, representing a sustainable strategy for exploiting waste and raw materials within a circular economy. Numerous reviews have compiled data on the methodology of marine collagen extraction, its application, as well as market and future prospects. However, this information is scattered across various publications, providing also an incomplete picture of the geographical distribution of the research and the species used. Therefore, the aim of this review is to conduct a systematic search to compile all relevant information. Additionally, we emphasize update legislation, providing recommendations, and offering future perspectives. The analysis showed that the majority of studies (72 percent) were conducted in Asia, primarily in China, followed by Europe and South America. Almost 60 percent of the species studied are marine, followed by aquaculture ones. The studies cover a wide geographical area, mainly focusing on species under high fishing pressure, needing sustainable management measures. Of the traditional extraction methods, enzymatic extraction produces the best results. However, this method is time- and energy-consuming. Green alternatives using supercritical fluids and deep eutectic solvents have emerged, with the expectation of reducing extraction times and higher yields. Further development is crucial for industrial-level exploitation, addressing economic challenges. Collagen is used in a wide range of products, with dietary supplements being its main application, particularly in view of the aging population. Meanwhile, the global collagen market is expected to continue growing exponentially while legislation lags behind, failing to adapt to developments.

Contribución INIDEP 2459
Lamas, Daniela; Alcolea Ersinger, Victoria F.; Massa, Agueda E. (2026).

Recovery, characterization and potential application as a detergent additive of viscera proteases from Longtail southern cod Patagonotothen ramsayi: a transition to circular economy. Waste and Biomass Valorization
https://doi.org/10.1007/s12649-025-03373-2

Abstract
Recently, efforts have been made to move the fishing industry towards smart fishing, enabling the long-term sustainability of the activity. Fish enzymes from viscera could be recycled and valorised for detergent use. This study aims to promote the application of a circular economy model using viscera from the southern long-tail cod (Patagonotothen ramsayi) fishery. Crude extracts of stomachs and intestines were purified using ammonium sulphate followed by membrane ultrafiltration. A full factorial design was used to select the conditions for the highest enzymatic activity, resulting in pH 8, 45 °C for 15 min and pH 2, 50 °C for 20 min for alkaline and acid proteases respectively. All activities were enhanced by the presence of CaCl2, Triton X-100, Tween 20 and Tween 80. However, SDS only had a positive effect on stomach proteinases. Acidic enzymes were stable in the presence of all surfactants tested, but their activity was not significantly improved. Conversely, the activity of all extracts was partially inhibited by EDTA. Compatibility with commercial detergents and washing tests performed showed positive effects. The information obtained from this work will increase the knowledge of the enzymes of this fishery to be used as an ingredient, which could have a triple impact benefit.

Contribución INIDEP 2390
Hop (Humulus lupulus) leaves as a functional ingredient to mitigate lipid oxidation and preserve PUFAs in rough scad patties

Mitton, F.M.; Turina, Y.; Kulisz, N.N.; Vittone, M.; Massa, A.E. (2025). Hop (Humulus lupulus) leaves as a functional ingredient to mitigate lipid oxidation and preserve PUFAs in rough scad patties. Food Chemistry Advances, 6. https://doi.org/10.1016/j.focha.2024.100865

Abstract
Fish products are rich in essential nutrients, especially polyunsaturated fatty acids (PUFAs), prone to lipid oxidation. This study explores hop (Humulus lupulus) leaves, a byproduct of the brewing industry, as a natural antioxidant in rough scad patties during storage conditions. Raw and cooked patties formulated with and without hop leaves or hop leaf extract were evaluated for macronutrient composition, fatty acid profile, amino acid content, phenolic compound content, and lipid peroxidation. The incorporation of hop leaves extract into raw patties resulted in a significant increase in phenolic compound content and antioxidant capacity. The protective properties of the extract were maintained in cooked patties, demonstrating its potential to preserve the nutritional value of PUFAs against lipid peroxidation. This research suggests that hop leaves could be a valuable natural source of antioxidants to mitigate lipid oxidation and preserve PUFAs in fish-based products.

Contribución INIDEP 2391
Culture of Bacillus subtilis and Saccharomyces cerevisiae from byproducts of the enzymatic extraction of Atlantoraja castelnaui oil

Salomone, A.L.; Lamas, D. (2025). Culture of Bacillus subtilis and Saccharomyces cerevisiae from byproducts of the enzymatic extraction of Atlantoraja castelnaui oil. Marine and Fishery Sciences, 38(2), 199-208. https://doi.org/10.47193/mafis.3822025010104

Abstract
The growing trend in global fish consumption has led to the generation of a large amount of waste and byproducts with negative economic and environmental impacts. The production of fish oil from fish processing residues seems to be a sustainable and future opportunity to provide valuable fatty acids for animal and human consumption. In parallel, this alternative valorization of fish waste has increased the demand to explore sustainable extraction methods. Enzymatic hydrolysis is an efficient, rapid and reproducible method for the extraction of oils from fish guts, producing an aqueous phase rich in proteins and soluble compounds. In this work, a highly soluble aqueous phase obtained from the enzymatic extraction of the liver oil of the ray Atlantoraja castelnaui was tested as a source of nutrient for the growth of Bacillus subtilis and Saccharomyces cerevisiae. Culture media were supplemented with the aqueous phase at a final concentration of 10 mg ml-1 total protein. The growth pattern and biomass yield of yeasts cultured in yeast extract peptone-dextrose (YPD) medium did not show statistically significant differences (p: 0.05) with the diluted medium and the aqueous fraction (AF) supplemented medium. Similar results were obtained for B. subtillis and its positive control in Luria Bertani (LB) medium. In both cases, the ability of these inexpensive media to support the growth of microorganisms was demonstrated. Results suggested that the remaining aqueous phase of the discards from A. castelnaui oil production can be used as an alternative substrate for microorganism culture purposes. In this way, a residue destined for disposal could become a product with added value achieving a good result in the context of circular economy.